Clinical Application of Regenerative Repair of Burn Wounds in Situ

According to the mechanism of in situ regenerative repair of wounds, burn regenerative therapy can be described as 8 technical procedures.

These 8 procedures should be followed in strict and standardized sequence and application. 

This is the foundation that BRT & MEBT/MEBO can achieve best clinical results.

procedure tissue organ replicaiton-enginneering

burn regeneration therapy, under the control of the 8 procedures can achieve surviving residual skin tissue repair and skin regeneration. The previoces figure not only explains the mechanism of the treatment of deep burn wounds of various causes, but also is applicable to the regenerative repair of various skin mucous membrane lesions and tissue organ injuries.

1. First of all, the PRCs in the deep layer of the injured skin tissue are activated to form stem cells: 

This is the start period of BRT The effect has a close collection with the treatment method. Early application of the drug to protect the wound surface and "Ploughing method" to reduce the tension of deep wounds can help to activate PRCs and skin organ regeneration in situ.

2. In situ cultivation of stem cells: 

The key point for this procedure is to maintain a physiologically moist environment created by MEBO and also protect the fibrous isolation membrane formed on the wound surface. This semi-permeable membrane allows the exchange of materials between both sides, from wound to MEBO.

3. Necrotic tissue should be liquefied and removed without any further damage, to create a condition for stem cell regeneration and repair in situ.

4. Nutrition supply: 

Stem cells proliferate veiy quickly and consume a lot of energy. Although there are enough nutritive materials for stem cells' growth in the body, when stem cells regenerate in an embryo development model, nutritive materials become veiy insufficient.

Exogenous and endogenous supply of the nutritive materials and physiological regulation are necessary. 

MEBO is a bionic nutritional preparation, its pH value and osmotic pressure are similar to that in the human body, it can provide all the necessary regenerative substances and nutrition for wound healing and regeneration and can bind stem cells, so continuous supply of MEBO will satisfy the nutrition supply of stem cell proliferation process.

5. Using physiological controlling technique to control bacterial and toxic infectious injuries is a non-bactericidal controlling technique: 

In the in situ stem cell cultivation technique, the treatment principle and method for microorganism in regenerative tissues is different with normal conception of sterility and technique of anti-bacteria. 

Stem cell and tissue regeneration is inhibited by antibiotics which are used in large doses in dry therapy. Thus, in order to prevent the damage of microorganism infection to regenerative cells, a non bactericidal controlling technique must be taken. 

MEBO can control bacterial infection and at the same time promote immunity of human body, so it has a comprehensive anti-infection effect.

6. To maintain a physiologically moist environment: 

All the wounds regardless of the location, a local wound or wounds all over the body, or wound in the gastrointestinal tract, need a physiologically moist environment.

Only in such an environment could skin regenerative repair be realized. This also includes the regulation and repair of dysfunction of the organs all over the body.

7.  Micro-isolation of burn wound and endogenous nutrition supply technique: 

The micro-isolation technique for skin tissue organ regeneration is realized by the "fibrous isolation membrane" together with "MEBO drug layer". 

Only after' setting up the micro-ecosystem, could the skin regeneration from stem cell in situ be fulfilled.

8. Tissue combination to form organs: 

After liquefaction and removal of necrotic tissue, wounds should be kept in a physiologically moist environment.

Tissues are combined without causing any damage, to form a capillary tree core and a fibrocyte trestle, so that skin stem cells can combine according to the normal physiological structure of the skin, to form primitive skin blastema and skin island.

MEBO should be supplied continuously to achieve full-thickness, physiological and regenerative repair of the skin tissue organs.