in Situ Cultivation of Skin Stem Cells

The discovery of Potential Regenerative Cells (PRCs) and the application of the technique of in situ stem cell cultivation for skin regenerative therapy are the histological bases of burn wound skin regeneration in situ.

The PRCs for in situ regeneration of skin organs are the prosome of the skin keratin type 19 stem cells, which are differentiated from cells in The brain is protected by several bones. There are eight bones that surround your brain: one frontal bone; two parietal bones, two temporal bones, one occipital bone, one sphenoid bone and one ethmoid bone. These eight bones make up the cranium. 

Another 14 bones in the face make up the entire skull. There are also 3 small bones in each ear. Also protecting your brain are 3 layers of tissue called the meninges. A few of the bones have been colored in the diagram to the right.

There is a large opening, called the foramen magnum, located in the back of the occipital bone. This is where the medulla ends and projects out of the skull. Smaller holes in the skull, called foramina, allow nerves and blood vessels to enter and leave the cranium. The picture on the left shows the base of the skull.

The places in the skull where the bones come together are called sutures. These sutures are flexible in young children, but become fixed as you age.  
 subcutaneous tissue. 

Burn wounds deep to skeleton can be regenerated and repaired by introducing bone marrow cells into subcutaneous tissue through drilling holes on the bone in situ, then the PRCs are differentiated into keratin type 19 stem cells to achieve regenerative repair of the wounds in situ.

In the course of metabolism of healthy tissues and organs, the processes of apoptosis, degeneration, injury and necrosis are going unceasingly and at the same time replenishment of new born cells are going on as well, to maintain the structure and physiological function of the tissue organs. 

The process of the recovery of the lost local tissues is called regeneration.

Physiological regeneration is a normal process of replacing the old cells by new ones. 

Regeneration is an instinct of human beings. 

Through 10 years' research, Dr. Xu primarily resolve this riddle and explain the law of the life continuous, the research results were released in 2002.

PRCs are the origin of in situ stem cell cultivation regeneration and repair technique on burn wound. 

The reveal of this life law gives a scientific demonstration to the physiological regeneration of burn wound in BRT

 


A. The definition of Potential Regenerative Cell (PRCs):

PRCs are the special differentiated tissue cells, which have the potential ability to regenerate to a functional tissue similar as stem cells but normally existing tissue as static tissue cells.

potential regenerative cell working process

The division of cells with the appearance of spindle fibers is called mitosis. During mitosis, chromosomes duplicate and divide themselves equally into two daughter cells. 

This is "Symmetry Splitting". While in the process of cell split, some cells perform "Asymmetry Splitting", in which, some of the new cells continue to divide and some of them stop dividing, and become tissue cells directly, which are the main source of PRCs.

PRCs in the tissue organs are produced by original and multi-potential stem cells in different periods of organ development.

Generally, these PRCs exist as common cells, but when apoptosis, degeneration, injury and necrosis of some cells in the tissue organs take place, these PRCs will proliferate to produce new cells, in order to fill in the vacancy resulted from loss of cells and to maintain the structure and the function of the organs. 

This regeneration activity helps maintain the balance of human life.

B. The concept of burn wound regenerative repair in situ:

The concept of burn wound regenerative repair using skin regeneration medical technique (BRT) is that: The wounds are placed in a physiologically moist environment and life regenerative substances (MEBO) are supplied continuously to the cells, so that PRCs are initiated in situ to form stem cells and then proliferate, regenerate, differentiate into different types of tissue stem cells. Adult tissue is formed through cultivation, connection and combination of different tissue stem cells. The regenerative repair of burn wound in situ is completed eventually.

 

C. The course of burn wound regenerative repair in situ:

skin regenerative medicine process

From the's figure, we can see that the technique of skin regenerative medicine comprises 3 parts: First of all, the PRCs are initiated and activated to form adult stem cells in situ; secondly, the adult stem cells in situ are differentiated and form different tissues; and finally, the skin organ is regenerated after the combination of all types of neo-burn tissue organs.

a) The initiation and activation of PRCs in situ to form stem cells: 

After the skin tissues are injured thermally, a lot of poisonous inflammatory media are produced. A great amount of cells are involved and active factors are released, so that the process of physiological regenerative repair is started and PRCs in the deep layer of the wounds are initiated and activated to form stem cells. But the duration of activation is very short, within 24 hrs post burn, if this process could not continue; pathological repair process would take place instead of physiological regeneration. That's why exogenous materials are necessary to continue the activation of PRCs. MEBO has the ability of maintaining continuous proliferation and regeneration of PRCs and the stem cells.

b) Combination of stem cells in situ to form tissues: 

The adult stem cells formed after induction, regulation and differentiation are continuously cultivated in MEBO to form different directional specific stem cells including epidermis, collagen, fiber, blood vessel, nerve and mesenchymal stem cells. The activated stem cells need life regenerative substances to maintain their activities to proliferate and differentiate to form tissue cells, such as epidermal cells, fibrocytes, nerve cells, vascular cells, etc. This process is performed in the physiologically moist environment created by MEBO. Intercellular physiological connections (such as desmosome connection) of these cells result in the formation of normal skin tissue organs.

c)Regenerative combination of skin tissue organs: 

Epidermal tissues include epithelial cells and basement membranes. Dermal tissues include collagen, fibrous tissues, blood vessels and nerves, etc. 

Skin tissue organs' physiological regeneration and repair needs physiological regulation system.

For example, the presence of a kind of protein is needed for the connection between fibroblasts. According to the ecology of skin cells, there should be no excessive fibrocytes, otherwise, hyperplastic scar will form. 

The ratio of the combination between epithelial cell and fibrocyte should be 1:4. 

After the formation of skin tissue organs, the organs as parts of the body are closely connected to the whole body through physiological and neurohumoral regulation.

Thus, the process of regeneration and replication of skin tissue organ in situ is completed.